Fig. 3. Atp6-CSTC substitutions restored the mitochondrial network in Tim11Δ cells but led to the aggregation of mitochondria in Om45-GFP cells expressing Tim11. A) Cells bearing plasmids expressing RFP fused to the mitochondrial targeting sequence were grown at 36 °C in liquid W0-GalA without leucine to an OD of 1 and immediately viewed by fluorescence microscopy. B) Morphology of mitochondria in cells grown in complete galactose medium at 36 °C. Defective mitochondrial network (A) or cristae invagination (B) is shown by arrows. Representative images are shown.